In this course, students will learn about fundamental molecular biology laboratory techniques and will use those techniques to carry out experiments which theoretically coincide with topics studied in MBGE302 – Molecular Biology I. Therefore, students should not take this lab course unless they have taken MBGE302 or are currently taking it. Experiments/techniques that will be discussed and carried out are; reverse transcription polymerase chain reactions (RT-PCR), single nucleotide polymorphism (SNP) analyses, immunoblotting or western blots, enzyme-linked immunosorbent assays (ELISA) and affinity chromatography. Additionally, cell/tissue cultures will be discussed and how to grow and maintain cell lines. Cells will be used to carry out metabolic experiments such as adenosine triphosphate assays, MTT assays for cell viability and TUNEL assays for apoptosis. Immunocytochemistry will also be covered.
- Teacher: Peyman Ince