In this course, students will learn about fundamental molecular biology laboratory techniques and will use those techniques to carry out experiments which theoretically coincide with topics studied in MBGE401 – Molecular Biology II. Therefore, students should not take this lab course unless they have taken MBGE401 or are currently taking it. Experiments/techniques that will be discussed and carried out are; the construction and cloning of recombinant DNA, DNA fingerprinting via Southern Blotting, and CRISPR. Additionally, cell lines will be used to discuss and carry out DNA and RNA extractions, cytotoxicity assays like LDH assays and protein extractions and protein concentration assays.
- Teacher: Peyman Ince